pet-15b vector (Millipore)
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pet-15b vector
Pet 15b Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pet-15b/pet28a/us12059418-269-19-21
Average 90 stars, based on 1 article reviews
Pet 15b Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pet-15b/pet28a/us12059418-269-19-21
Average 90 stars, based on 1 article reviews
pet-15b vector - by Bioz Stars,
2026-10
90/100 stars
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Purification:Article Title: SseA is a chaperone for the SseB and SseD translocon components of the Salmonella pathogenicity-island-2-encoded type III secretion system. Article Snippet: Received 16 December 2002 Revised 27 January 2003 Accepted 29 January 2003 The type III secretion system (TTSS) encoded by the Salmonella pathogenicity island 2 (SPI-2) is required for bacterial replication inside macrophages and for systemic infection in mice.. Many TTSS secreted proteins, including effectors and components of the translocon, require chaperones which promote their stability, prevent their premature interactions or facilitate their secretion.. In this study, the function of the first gene (sseA) of one of the SPI-2 operons (sseA–G) was investigated. Article Title: Virtual screening using ligand-based pharmacophores for inhibitors of human tyrosyl-DNA phospodiesterase (hTdp1) Article Snippet: Preparation of human Tdp1 Human Tdp1 expressing plasmid pHN1910 (a gift from Dr. Howard Nash, Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health) was constructed using Article Title: Accelerating the discovery of alkyl halide-derived natural products using halide depletion. Article Snippet: Article Title: PKR and GCN2 Kinases and Guanine Nucleotide Exchange Factor Eukaryotic Translation Initiation Factor 2B (eIF2B) Recognize Overlapping Surfaces on eIF2α Article Snippet: Article Title: Characterization of an α-L-fucosidase in marine bacterium Wenyingzhuangia fucanilytica: new evidence on the catalytic sites of GH95 family glycosidases. Article Snippet: Background: ⊍-L-Fucose confers unique functions for fucose-containing biomolecules such as humanmilk oligosaccharides.. ⊍-LFucosidases can serve as desirable tools in the application of fucosylated saccharides.. Discovering novel ⊍-L-fucosidases and elucidating their enzyme properties are always worthy tasks. Article Title: NLS copy-number variation governs efficiency of nuclear import--case study on dUTPases. Article Snippet: The D. virilis homotrimeric AAA dUTPase was constructed by amplifying the A subunit of the ABC pseudo-heterotrimer from genomic DNA using primers DvirDF and DvirDRs1, and the fragment was cloned into the NheI/BglII sites of Article Title: Human Nbp35 Is Essential for both Cytosolic Iron-Sulfur Protein Assembly and Iron Homeostasis Article Snippet: Proteins were expressed in Escherichia coli in the presence of the bacterial isc operon using Article Title: Crystal structure and mutational analysis of the human TRIM7 B30.2 domain provide insights into the molecular basis of its binding to glycogenin-1 Article Snippet: Escherichia coli strain Rosetta(DE3) and vector pET-15b were purchased from Novagen. pTYB11 vector and chitin agarose beads were obtained from New England Biolabs. Polymerase Chain Reaction:Article Title: SseA is a chaperone for the SseB and SseD translocon components of the Salmonella pathogenicity-island-2-encoded type III secretion system. Article Snippet: Received 16 December 2002 Revised 27 January 2003 Accepted 29 January 2003 The type III secretion system (TTSS) encoded by the Salmonella pathogenicity island 2 (SPI-2) is required for bacterial replication inside macrophages and for systemic infection in mice.. Many TTSS secreted proteins, including effectors and components of the translocon, require chaperones which promote their stability, prevent their premature interactions or facilitate their secretion.. In this study, the function of the first gene (sseA) of one of the SPI-2 operons (sseA–G) was investigated. Article Title: Virtual screening using ligand-based pharmacophores for inhibitors of human tyrosyl-DNA phospodiesterase (hTdp1) Article Snippet: Preparation of human Tdp1 Human Tdp1 expressing plasmid pHN1910 (a gift from Dr. Howard Nash, Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health) was constructed using Article Title: Accelerating the discovery of alkyl halide-derived natural products using halide depletion. Article Snippet: Article Title: PKR and GCN2 Kinases and Guanine Nucleotide Exchange Factor Eukaryotic Translation Initiation Factor 2B (eIF2B) Recognize Overlapping Surfaces on eIF2α Article Snippet: Article Title: Characterization of an α-L-fucosidase in marine bacterium Wenyingzhuangia fucanilytica: new evidence on the catalytic sites of GH95 family glycosidases. Article Snippet: Background: ⊍-L-Fucose confers unique functions for fucose-containing biomolecules such as humanmilk oligosaccharides.. ⊍-LFucosidases can serve as desirable tools in the application of fucosylated saccharides.. Discovering novel ⊍-L-fucosidases and elucidating their enzyme properties are always worthy tasks. Article Title: NLS copy-number variation governs efficiency of nuclear import--case study on dUTPases. Article Snippet: The D. virilis homotrimeric AAA dUTPase was constructed by amplifying the A subunit of the ABC pseudo-heterotrimer from genomic DNA using primers DvirDF and DvirDRs1, and the fragment was cloned into the NheI/BglII sites of Article Title: Human Nbp35 Is Essential for both Cytosolic Iron-Sulfur Protein Assembly and Iron Homeostasis Article Snippet: Proteins were expressed in Escherichia coli in the presence of the bacterial isc operon using Article Title: Crystal structure and mutational analysis of the human TRIM7 B30.2 domain provide insights into the molecular basis of its binding to glycogenin-1 Article Snippet: Escherichia coli strain Rosetta(DE3) and vector pET-15b were purchased from Novagen. pTYB11 vector and chitin agarose beads were obtained from New England Biolabs. Clone Assay:Article Title: SseA is a chaperone for the SseB and SseD translocon components of the Salmonella pathogenicity-island-2-encoded type III secretion system. Article Snippet: Received 16 December 2002 Revised 27 January 2003 Accepted 29 January 2003 The type III secretion system (TTSS) encoded by the Salmonella pathogenicity island 2 (SPI-2) is required for bacterial replication inside macrophages and for systemic infection in mice.. Many TTSS secreted proteins, including effectors and components of the translocon, require chaperones which promote their stability, prevent their premature interactions or facilitate their secretion.. In this study, the function of the first gene (sseA) of one of the SPI-2 operons (sseA–G) was investigated. Article Title: Virtual screening using ligand-based pharmacophores for inhibitors of human tyrosyl-DNA phospodiesterase (hTdp1) Article Snippet: Preparation of human Tdp1 Human Tdp1 expressing plasmid pHN1910 (a gift from Dr. Howard Nash, Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health) was constructed using Article Title: Accelerating the discovery of alkyl halide-derived natural products using halide depletion. Article Snippet: Article Title: PKR and GCN2 Kinases and Guanine Nucleotide Exchange Factor Eukaryotic Translation Initiation Factor 2B (eIF2B) Recognize Overlapping Surfaces on eIF2α Article Snippet: Article Title: Characterization of an α-L-fucosidase in marine bacterium Wenyingzhuangia fucanilytica: new evidence on the catalytic sites of GH95 family glycosidases. Article Snippet: Background: ⊍-L-Fucose confers unique functions for fucose-containing biomolecules such as humanmilk oligosaccharides.. ⊍-LFucosidases can serve as desirable tools in the application of fucosylated saccharides.. Discovering novel ⊍-L-fucosidases and elucidating their enzyme properties are always worthy tasks. Article Title: NLS copy-number variation governs efficiency of nuclear import--case study on dUTPases. Article Snippet: The D. virilis homotrimeric AAA dUTPase was constructed by amplifying the A subunit of the ABC pseudo-heterotrimer from genomic DNA using primers DvirDF and DvirDRs1, and the fragment was cloned into the NheI/BglII sites of Article Title: Human Nbp35 Is Essential for both Cytosolic Iron-Sulfur Protein Assembly and Iron Homeostasis Article Snippet: Proteins were expressed in Escherichia coli in the presence of the bacterial isc operon using Article Title: Crystal structure and mutational analysis of the human TRIM7 B30.2 domain provide insights into the molecular basis of its binding to glycogenin-1 Article Snippet: Escherichia coli strain Rosetta(DE3) and vector pET-15b were purchased from Novagen. pTYB11 vector and chitin agarose beads were obtained from New England Biolabs. Plasmid Preparation:Article Title: SseA is a chaperone for the SseB and SseD translocon components of the Salmonella pathogenicity-island-2-encoded type III secretion system. Article Snippet: Received 16 December 2002 Revised 27 January 2003 Accepted 29 January 2003 The type III secretion system (TTSS) encoded by the Salmonella pathogenicity island 2 (SPI-2) is required for bacterial replication inside macrophages and for systemic infection in mice.. Many TTSS secreted proteins, including effectors and components of the translocon, require chaperones which promote their stability, prevent their premature interactions or facilitate their secretion.. In this study, the function of the first gene (sseA) of one of the SPI-2 operons (sseA–G) was investigated. Article Title: Virtual screening using ligand-based pharmacophores for inhibitors of human tyrosyl-DNA phospodiesterase (hTdp1) Article Snippet: Preparation of human Tdp1 Human Tdp1 expressing plasmid pHN1910 (a gift from Dr. Howard Nash, Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health) was constructed using Article Title: Accelerating the discovery of alkyl halide-derived natural products using halide depletion. Article Snippet: Article Title: PKR and GCN2 Kinases and Guanine Nucleotide Exchange Factor Eukaryotic Translation Initiation Factor 2B (eIF2B) Recognize Overlapping Surfaces on eIF2α Article Snippet: Article Title: Characterization of an α-L-fucosidase in marine bacterium Wenyingzhuangia fucanilytica: new evidence on the catalytic sites of GH95 family glycosidases. Article Snippet: Background: ⊍-L-Fucose confers unique functions for fucose-containing biomolecules such as humanmilk oligosaccharides.. ⊍-LFucosidases can serve as desirable tools in the application of fucosylated saccharides.. Discovering novel ⊍-L-fucosidases and elucidating their enzyme properties are always worthy tasks. Article Title: NLS copy-number variation governs efficiency of nuclear import--case study on dUTPases. Article Snippet: The D. virilis homotrimeric AAA dUTPase was constructed by amplifying the A subunit of the ABC pseudo-heterotrimer from genomic DNA using primers DvirDF and DvirDRs1, and the fragment was cloned into the NheI/BglII sites of Article Title: Human Nbp35 Is Essential for both Cytosolic Iron-Sulfur Protein Assembly and Iron Homeostasis Article Snippet: Proteins were expressed in Escherichia coli in the presence of the bacterial isc operon using Article Title: Crystal structure and mutational analysis of the human TRIM7 B30.2 domain provide insights into the molecular basis of its binding to glycogenin-1 Article Snippet: Escherichia coli strain Rosetta(DE3) and vector pET-15b were purchased from Novagen. pTYB11 vector and chitin agarose beads were obtained from New England Biolabs. Construct:Article Title: SseA is a chaperone for the SseB and SseD translocon components of the Salmonella pathogenicity-island-2-encoded type III secretion system. Article Snippet: Received 16 December 2002 Revised 27 January 2003 Accepted 29 January 2003 The type III secretion system (TTSS) encoded by the Salmonella pathogenicity island 2 (SPI-2) is required for bacterial replication inside macrophages and for systemic infection in mice.. Many TTSS secreted proteins, including effectors and components of the translocon, require chaperones which promote their stability, prevent their premature interactions or facilitate their secretion.. In this study, the function of the first gene (sseA) of one of the SPI-2 operons (sseA–G) was investigated. Article Title: Virtual screening using ligand-based pharmacophores for inhibitors of human tyrosyl-DNA phospodiesterase (hTdp1) Article Snippet: Preparation of human Tdp1 Human Tdp1 expressing plasmid pHN1910 (a gift from Dr. Howard Nash, Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health) was constructed using Article Title: Accelerating the discovery of alkyl halide-derived natural products using halide depletion. Article Snippet: Article Title: PKR and GCN2 Kinases and Guanine Nucleotide Exchange Factor Eukaryotic Translation Initiation Factor 2B (eIF2B) Recognize Overlapping Surfaces on eIF2α Article Snippet: Article Title: Characterization of an α-L-fucosidase in marine bacterium Wenyingzhuangia fucanilytica: new evidence on the catalytic sites of GH95 family glycosidases. Article Snippet: Background: ⊍-L-Fucose confers unique functions for fucose-containing biomolecules such as humanmilk oligosaccharides.. ⊍-LFucosidases can serve as desirable tools in the application of fucosylated saccharides.. Discovering novel ⊍-L-fucosidases and elucidating their enzyme properties are always worthy tasks. Article Title: NLS copy-number variation governs efficiency of nuclear import--case study on dUTPases. Article Snippet: The D. virilis homotrimeric AAA dUTPase was constructed by amplifying the A subunit of the ABC pseudo-heterotrimer from genomic DNA using primers DvirDF and DvirDRs1, and the fragment was cloned into the NheI/BglII sites of Article Title: Human Nbp35 Is Essential for both Cytosolic Iron-Sulfur Protein Assembly and Iron Homeostasis Article Snippet: Proteins were expressed in Escherichia coli in the presence of the bacterial isc operon using Article Title: Crystal structure and mutational analysis of the human TRIM7 B30.2 domain provide insights into the molecular basis of its binding to glycogenin-1 Article Snippet: Escherichia coli strain Rosetta(DE3) and vector pET-15b were purchased from Novagen. pTYB11 vector and chitin agarose beads were obtained from New England Biolabs. Expressing:Article Title: SseA is a chaperone for the SseB and SseD translocon components of the Salmonella pathogenicity-island-2-encoded type III secretion system. Article Snippet: Received 16 December 2002 Revised 27 January 2003 Accepted 29 January 2003 The type III secretion system (TTSS) encoded by the Salmonella pathogenicity island 2 (SPI-2) is required for bacterial replication inside macrophages and for systemic infection in mice.. Many TTSS secreted proteins, including effectors and components of the translocon, require chaperones which promote their stability, prevent their premature interactions or facilitate their secretion.. In this study, the function of the first gene (sseA) of one of the SPI-2 operons (sseA–G) was investigated. Article Title: Virtual screening using ligand-based pharmacophores for inhibitors of human tyrosyl-DNA phospodiesterase (hTdp1) Article Snippet: Preparation of human Tdp1 Human Tdp1 expressing plasmid pHN1910 (a gift from Dr. Howard Nash, Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health) was constructed using Article Title: Accelerating the discovery of alkyl halide-derived natural products using halide depletion. Article Snippet: Article Title: PKR and GCN2 Kinases and Guanine Nucleotide Exchange Factor Eukaryotic Translation Initiation Factor 2B (eIF2B) Recognize Overlapping Surfaces on eIF2α Article Snippet: Article Title: Characterization of an α-L-fucosidase in marine bacterium Wenyingzhuangia fucanilytica: new evidence on the catalytic sites of GH95 family glycosidases. Article Snippet: Background: ⊍-L-Fucose confers unique functions for fucose-containing biomolecules such as humanmilk oligosaccharides.. ⊍-LFucosidases can serve as desirable tools in the application of fucosylated saccharides.. Discovering novel ⊍-L-fucosidases and elucidating their enzyme properties are always worthy tasks. Article Title: NLS copy-number variation governs efficiency of nuclear import--case study on dUTPases. Article Snippet: The D. virilis homotrimeric AAA dUTPase was constructed by amplifying the A subunit of the ABC pseudo-heterotrimer from genomic DNA using primers DvirDF and DvirDRs1, and the fragment was cloned into the NheI/BglII sites of Article Title: Human Nbp35 Is Essential for both Cytosolic Iron-Sulfur Protein Assembly and Iron Homeostasis Article Snippet: Proteins were expressed in Escherichia coli in the presence of the bacterial isc operon using Article Title: Crystal structure and mutational analysis of the human TRIM7 B30.2 domain provide insights into the molecular basis of its binding to glycogenin-1 Article Snippet: Escherichia coli strain Rosetta(DE3) and vector pET-15b were purchased from Novagen. pTYB11 vector and chitin agarose beads were obtained from New England Biolabs. Sequencing:Article Title: SseA is a chaperone for the SseB and SseD translocon components of the Salmonella pathogenicity-island-2-encoded type III secretion system. Article Snippet: Received 16 December 2002 Revised 27 January 2003 Accepted 29 January 2003 The type III secretion system (TTSS) encoded by the Salmonella pathogenicity island 2 (SPI-2) is required for bacterial replication inside macrophages and for systemic infection in mice.. Many TTSS secreted proteins, including effectors and components of the translocon, require chaperones which promote their stability, prevent their premature interactions or facilitate their secretion.. In this study, the function of the first gene (sseA) of one of the SPI-2 operons (sseA–G) was investigated. Article Title: Virtual screening using ligand-based pharmacophores for inhibitors of human tyrosyl-DNA phospodiesterase (hTdp1) Article Snippet: Preparation of human Tdp1 Human Tdp1 expressing plasmid pHN1910 (a gift from Dr. Howard Nash, Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health) was constructed using Article Title: Accelerating the discovery of alkyl halide-derived natural products using halide depletion. Article Snippet: Article Title: PKR and GCN2 Kinases and Guanine Nucleotide Exchange Factor Eukaryotic Translation Initiation Factor 2B (eIF2B) Recognize Overlapping Surfaces on eIF2α Article Snippet: Article Title: Characterization of an α-L-fucosidase in marine bacterium Wenyingzhuangia fucanilytica: new evidence on the catalytic sites of GH95 family glycosidases. Article Snippet: Background: ⊍-L-Fucose confers unique functions for fucose-containing biomolecules such as humanmilk oligosaccharides.. ⊍-LFucosidases can serve as desirable tools in the application of fucosylated saccharides.. Discovering novel ⊍-L-fucosidases and elucidating their enzyme properties are always worthy tasks. Article Title: NLS copy-number variation governs efficiency of nuclear import--case study on dUTPases. Article Snippet: The D. virilis homotrimeric AAA dUTPase was constructed by amplifying the A subunit of the ABC pseudo-heterotrimer from genomic DNA using primers DvirDF and DvirDRs1, and the fragment was cloned into the NheI/BglII sites of Article Title: Human Nbp35 Is Essential for both Cytosolic Iron-Sulfur Protein Assembly and Iron Homeostasis Article Snippet: Proteins were expressed in Escherichia coli in the presence of the bacterial isc operon using Article Title: Crystal structure and mutational analysis of the human TRIM7 B30.2 domain provide insights into the molecular basis of its binding to glycogenin-1 Article Snippet: Escherichia coli strain Rosetta(DE3) and vector pET-15b were purchased from Novagen. pTYB11 vector and chitin agarose beads were obtained from New England Biolabs. Affinity Chromatography:Article Title: SseA is a chaperone for the SseB and SseD translocon components of the Salmonella pathogenicity-island-2-encoded type III secretion system. Article Snippet: Received 16 December 2002 Revised 27 January 2003 Accepted 29 January 2003 The type III secretion system (TTSS) encoded by the Salmonella pathogenicity island 2 (SPI-2) is required for bacterial replication inside macrophages and for systemic infection in mice.. Many TTSS secreted proteins, including effectors and components of the translocon, require chaperones which promote their stability, prevent their premature interactions or facilitate their secretion.. In this study, the function of the first gene (sseA) of one of the SPI-2 operons (sseA–G) was investigated. Article Title: Virtual screening using ligand-based pharmacophores for inhibitors of human tyrosyl-DNA phospodiesterase (hTdp1) Article Snippet: Preparation of human Tdp1 Human Tdp1 expressing plasmid pHN1910 (a gift from Dr. Howard Nash, Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health) was constructed using Article Title: Accelerating the discovery of alkyl halide-derived natural products using halide depletion. Article Snippet: Article Title: PKR and GCN2 Kinases and Guanine Nucleotide Exchange Factor Eukaryotic Translation Initiation Factor 2B (eIF2B) Recognize Overlapping Surfaces on eIF2α Article Snippet: Article Title: Characterization of an α-L-fucosidase in marine bacterium Wenyingzhuangia fucanilytica: new evidence on the catalytic sites of GH95 family glycosidases. Article Snippet: Background: ⊍-L-Fucose confers unique functions for fucose-containing biomolecules such as humanmilk oligosaccharides.. ⊍-LFucosidases can serve as desirable tools in the application of fucosylated saccharides.. Discovering novel ⊍-L-fucosidases and elucidating their enzyme properties are always worthy tasks. Article Title: NLS copy-number variation governs efficiency of nuclear import--case study on dUTPases. Article Snippet: The D. virilis homotrimeric AAA dUTPase was constructed by amplifying the A subunit of the ABC pseudo-heterotrimer from genomic DNA using primers DvirDF and DvirDRs1, and the fragment was cloned into the NheI/BglII sites of Article Title: Human Nbp35 Is Essential for both Cytosolic Iron-Sulfur Protein Assembly and Iron Homeostasis Article Snippet: Proteins were expressed in Escherichia coli in the presence of the bacterial isc operon using Article Title: Crystal structure and mutational analysis of the human TRIM7 B30.2 domain provide insights into the molecular basis of its binding to glycogenin-1 Article Snippet: Escherichia coli strain Rosetta(DE3) and vector pET-15b were purchased from Novagen. pTYB11 vector and chitin agarose beads were obtained from New England Biolabs. Generated:Article Title: SseA is a chaperone for the SseB and SseD translocon components of the Salmonella pathogenicity-island-2-encoded type III secretion system. Article Snippet: Received 16 December 2002 Revised 27 January 2003 Accepted 29 January 2003 The type III secretion system (TTSS) encoded by the Salmonella pathogenicity island 2 (SPI-2) is required for bacterial replication inside macrophages and for systemic infection in mice.. Many TTSS secreted proteins, including effectors and components of the translocon, require chaperones which promote their stability, prevent their premature interactions or facilitate their secretion.. In this study, the function of the first gene (sseA) of one of the SPI-2 operons (sseA–G) was investigated. Article Title: Virtual screening using ligand-based pharmacophores for inhibitors of human tyrosyl-DNA phospodiesterase (hTdp1) Article Snippet: Preparation of human Tdp1 Human Tdp1 expressing plasmid pHN1910 (a gift from Dr. Howard Nash, Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health) was constructed using Article Title: Accelerating the discovery of alkyl halide-derived natural products using halide depletion. Article Snippet: Article Title: PKR and GCN2 Kinases and Guanine Nucleotide Exchange Factor Eukaryotic Translation Initiation Factor 2B (eIF2B) Recognize Overlapping Surfaces on eIF2α Article Snippet: Article Title: Characterization of an α-L-fucosidase in marine bacterium Wenyingzhuangia fucanilytica: new evidence on the catalytic sites of GH95 family glycosidases. Article Snippet: Background: ⊍-L-Fucose confers unique functions for fucose-containing biomolecules such as humanmilk oligosaccharides.. ⊍-LFucosidases can serve as desirable tools in the application of fucosylated saccharides.. Discovering novel ⊍-L-fucosidases and elucidating their enzyme properties are always worthy tasks. Article Title: NLS copy-number variation governs efficiency of nuclear import--case study on dUTPases. Article Snippet: The D. virilis homotrimeric AAA dUTPase was constructed by amplifying the A subunit of the ABC pseudo-heterotrimer from genomic DNA using primers DvirDF and DvirDRs1, and the fragment was cloned into the NheI/BglII sites of Article Title: Human Nbp35 Is Essential for both Cytosolic Iron-Sulfur Protein Assembly and Iron Homeostasis Article Snippet: Proteins were expressed in Escherichia coli in the presence of the bacterial isc operon using Article Title: Crystal structure and mutational analysis of the human TRIM7 B30.2 domain provide insights into the molecular basis of its binding to glycogenin-1 Article Snippet: Escherichia coli strain Rosetta(DE3) and vector pET-15b were purchased from Novagen. pTYB11 vector and chitin agarose beads were obtained from New England Biolabs. Subcloning:Article Title: SseA is a chaperone for the SseB and SseD translocon components of the Salmonella pathogenicity-island-2-encoded type III secretion system. Article Snippet: Received 16 December 2002 Revised 27 January 2003 Accepted 29 January 2003 The type III secretion system (TTSS) encoded by the Salmonella pathogenicity island 2 (SPI-2) is required for bacterial replication inside macrophages and for systemic infection in mice.. Many TTSS secreted proteins, including effectors and components of the translocon, require chaperones which promote their stability, prevent their premature interactions or facilitate their secretion.. In this study, the function of the first gene (sseA) of one of the SPI-2 operons (sseA–G) was investigated. Article Title: Virtual screening using ligand-based pharmacophores for inhibitors of human tyrosyl-DNA phospodiesterase (hTdp1) Article Snippet: Preparation of human Tdp1 Human Tdp1 expressing plasmid pHN1910 (a gift from Dr. Howard Nash, Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health) was constructed using Article Title: Accelerating the discovery of alkyl halide-derived natural products using halide depletion. Article Snippet: Article Title: PKR and GCN2 Kinases and Guanine Nucleotide Exchange Factor Eukaryotic Translation Initiation Factor 2B (eIF2B) Recognize Overlapping Surfaces on eIF2α Article Snippet: Article Title: Characterization of an α-L-fucosidase in marine bacterium Wenyingzhuangia fucanilytica: new evidence on the catalytic sites of GH95 family glycosidases. Article Snippet: Background: ⊍-L-Fucose confers unique functions for fucose-containing biomolecules such as humanmilk oligosaccharides.. ⊍-LFucosidases can serve as desirable tools in the application of fucosylated saccharides.. Discovering novel ⊍-L-fucosidases and elucidating their enzyme properties are always worthy tasks. Article Title: NLS copy-number variation governs efficiency of nuclear import--case study on dUTPases. Article Snippet: The D. virilis homotrimeric AAA dUTPase was constructed by amplifying the A subunit of the ABC pseudo-heterotrimer from genomic DNA using primers DvirDF and DvirDRs1, and the fragment was cloned into the NheI/BglII sites of Article Title: Human Nbp35 Is Essential for both Cytosolic Iron-Sulfur Protein Assembly and Iron Homeostasis Article Snippet: Proteins were expressed in Escherichia coli in the presence of the bacterial isc operon using Article Title: Crystal structure and mutational analysis of the human TRIM7 B30.2 domain provide insights into the molecular basis of its binding to glycogenin-1 Article Snippet: Escherichia coli strain Rosetta(DE3) and vector pET-15b were purchased from Novagen. pTYB11 vector and chitin agarose beads were obtained from New England Biolabs. |